› 5vkt

X-ray diffraction
1.83Å resolution

Cinnamyl alcohol dehydrogenases (SbCAD4) from Sorghum bicolor (L.) Moench

Released:
DOI: 10.2210/pdb5vkt/pdb
PDB entry 5vkt coloured by chain, front view.
PDB entry 5vkt coloured by chain, side view.
PDB entry 5vkt coloured by chain, top view.
Source organism: Sorghum bicolor
Primary publication:
The Enzyme Activity and Substrate Specificity of Two Major Cinnamyl Alcohol Dehydrogenases in Sorghum (Sorghum bicolor), SbCAD2 and SbCAD4.
Jun SY, Walker AM, Kim H, Ralph J, Vermerris W, Sattler SE, Kang C
Plant Physiol 174 2128-2145 (2017)
PMID: 28606901

Function and Biology Details

Reaction catalysed: 
Cinnamyl alcohol + NADP(+) = cinnamaldehyde + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-504269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cinnamyl-alcohol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 37.93 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: C5XC49 (Residues: 1-362; Coverage: 100%)
Gene name: SORBI_3002G195400
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
5 bound ligands:
Ligand ZN 4 x ZN
Ligand GOL 1 x GOL
Ligand FMT 1 x FMT
Ligand MLT 1 x MLT
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 55.597Å b: 74.28Å c: 97.288Å
α: 90° β: 96.74° γ: 90°
R-values:
R R work R free
0.164 0.163 0.187
Expression system: Escherichia coli

Quick links

Citations

12 citation in other articles

Natural variation in ZmFBL41 confers banded leaf and sheath blight resistance in maize.
Li et al. (2019)
11 more

1 mention without citation

Structural Basis for Broad Substrate Selectivity of Alcohol Dehydrogenase YjgB from Escherichia coli.
Nguyen et al. (2020)