› 5vlg

X-ray diffraction
2.93Å resolution

Crystal structure of EilR in complex with malachite green

Released:
DOI: 10.2210/pdb5vlg/pdb
PDB entry 5vlg coloured by chain, front view.
PDB entry 5vlg coloured by chain, side view.
PDB entry 5vlg coloured by chain, top view.
Source organism: [Enterobacter] lignolyticus
Primary publication:
Jungle Express is a versatile repressor system for tight transcriptional control.
Ruegg TL, Pereira JH, Chen JC, DeGiovanni A, Novichkov P, Mutalik VK, Tomaleri GP, Singer SW, Hillson NJ, Simmons BA, Adams PD, Thelen MP
OpenAccess logo Nat Commun 9 3617 (2018)
PMID: 30190458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 192 amino acids
Theoretical weight: 21.71 KDa
Source organism: [Enterobacter] lignolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: E3G817 (Residues: 1-192; Coverage: 100%)
Gene names: Entcl_2353, eilR
Sequence domains: Bacterial regulatory proteins, tetR family

Ligands and Environments

1 bound ligand:
Ligand MGR 8 x MGR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 100.145Å b: 85.985Å c: 102.275Å
α: 90° β: 101.69° γ: 90°
R-values:
R R work R free
0.199 0.197 0.24
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Microbial production of advanced biofuels.
Keasling et al. (2021)
2 more