› 5vo5

X-ray diffraction
2Å resolution

Crystal structure of Lgd-Shrub complex, single chain fusion

Released:
DOI: 10.2210/pdb5vo5/pdb
PDB entry 5vo5 coloured by chain, front view.
PDB entry 5vo5 coloured by chain, side view.
PDB entry 5vo5 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural Basis for Regulation of ESCRT-III Complexes by Lgd.
McMillan BJ, Tibbe C, Drabek AA, Seegar TCM, Blacklow SC, Klein T
Cell Rep 19 1750-1757 (2017)
PMID: 28564595

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-570095 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Charged multivesicular body protein 4; Coiled-coil and C2 domain-containing protein 1-like Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 20.91 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VKJ9 (Residues: 359-423; Coverage: 8%)
  • Canonical: Q8T0Q4 (Residues: 17-130; Coverage: 50%)
Gene names: CG4713, CG8055, Shrub, Snf7, Vps32, l(2)gd1, lgd, shrb
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 120.97Å b: 26.99Å c: 82.84Å
α: 90° β: 125.95° γ: 90°
R-values:
R R work R free
0.243 0.24 0.269
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structures, Functions, and Dynamics of ESCRT-III/Vps4 Membrane Remodeling and Fission Complexes.
McCullough et al. (2018)
1 more

2 mentions without citation

Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex.
Butt et al. (2020)
1 more