› 5vsz

X-ray diffraction
2.4Å resolution

Structure of the Ubl domain of Sacsin mutant L78M

Released:
DOI: 10.2210/pdb5vsz/pdb
PDB entry 5vsz coloured by chain, front view.
PDB entry 5vsz coloured by chain, side view.
PDB entry 5vsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations.
Ménade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S, Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young JC, Gehring K
J Biol Chem 293 12832-12842 (2018)
PMID: 29945973

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sacsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 89 amino acids
Theoretical weight: 9.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZJ4 (Residues: 2-85; Coverage: 2%)
Gene names: DNAJC29, KIAA0730, SACS
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2221
Unit cell:
a: 52.14Å b: 61.45Å c: 91.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.218 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Classification of Autosomal Recessive Cerebellar Ataxias: a Consensus Statement from the Society for Research on the Cerebellum and Ataxias Task Force.
Beaudin et al. (2019)
2 more

1 mention without citation

Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations.
Ménade et al. (2018)