› 5vws

X-ray diffraction
2.41Å resolution

Ligand free structure of Cytochrome P450 TbtJ1

Released:
DOI: 10.2210/pdb5vws/pdb
PDB entry 5vws coloured by chain, front view.
PDB entry 5vws coloured by chain, side view.
PDB entry 5vws coloured by chain, top view.
Source organism: Thermobispora bispora DSM 43833
Primary publication:
P450-Mediated Non-natural Cyclopropanation of Dehydroalanine-Containing Thiopeptides.
Gober JG, Ghodge SV, Bogart JW, Wever WJ, Watkins RR, Brustad EM, Bowers AA
ACS Chem Biol 12 1726-1731 (2017)
PMID: 28535034

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-505547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 44.66 KDa
Source organism: Thermobispora bispora DSM 43833
Expression system: Escherichia coli
UniProt:
  • Canonical: D6Y4Z8 (Residues: 1-390; Coverage: 100%)
Gene name: Tbis_0546
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 95.316Å b: 95.316Å c: 126.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.281
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Biocatalytic Oxidation Reactions: A Chemist's Perspective.
Dong et al. (2018)
8 more

3 mentions without citation

Clustering of Aromatic Amino Acid Residues around Methionine in Proteins.
Gibbs et al. (2021)
2 more