› 5vy9

Electron Microscopy
6.7Å resolution

S. cerevisiae Hsp104:casein complex, Middle Domain Conformation

Released:
DOI: 10.2210/pdb5vy9/pdb
PDB entry 5vy9 coloured by chain, front view.
PDB entry 5vy9 coloured by chain, side view.
PDB entry 5vy9 coloured by chain, top view.
Source organisms:
Primary publication:
Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104.
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science 357 273-279 (2017)
PMID: 28619716
Related structures: EMD-8745

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152030 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock protein 104 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 908 amino acids
Theoretical weight: 102.17 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P31539 (Residues: 1-908; Coverage: 100%)
Gene names: HSP104, L0948, YLL026W
Sequence domains:
Alpha-S1-casein Chain: P
Molecule details ›
Chain: P
Length: 28 amino acids
Theoretical weight: 2.4 KDa
Source organism: Bos taurus

Ligands and Environments

1 bound ligand:
Ligand AGS 12 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.7Å
Relevant EMDB volumes: EMD-8745
Expression system: Escherichia coli

Quick links

Citations

43 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
42 more

8 mentions without citation

A History of Molecular Chaperone Structures in the Protein Data Bank.
Bascos et al. (2019)
7 more