› 5vyu

X-ray diffraction
2.2Å resolution

Crystal structure of the WbkC N-formyltransferase from Brucella melitensis in complex with GDP-perosaminea and N-10-formyltetrahydrofolate

Released:
DOI: 10.2210/pdb5vyu/pdb
PDB entry 5vyu coloured by chain, front view.
PDB entry 5vyu coloured by chain, side view.
PDB entry 5vyu coloured by chain, top view.
Source organism: Brucella melitensis bv. 1 str. 16M
Primary publication:
Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis.
Riegert AS, Chantigian DP, Thoden JB, Tipton PA, Holden HM
Biochemistry 56 3657-3668 (2017)
PMID: 28636341

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + GDP-alpha-D-perosamine = tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-perosamine N-formyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 29.24 KDa
Source organism: Brucella melitensis bv. 1 str. 16M
Expression system: Escherichia coli
UniProt:
  • Canonical: F8WJP6 (Residues: 1-259; Coverage: 100%)
Gene names: BAWG_0804, BMEI1418, wbkC
Sequence domains: Formyl transferase

Ligands and Environments

3 bound ligands:
Ligand 1YA 2 x 1YA
Ligand JB2 1 x JB2
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 66.835Å b: 66.835Å c: 235.947Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.264
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Investigation of a sugar N-formyltransferase from the plant pathogen Pantoea ananatis.
Hofmeister et al. (2019)
3 more

1 mention without citation

Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis.
Riegert et al. (2017)