› 5w1d

X-ray diffraction
3.35Å resolution

Crystal Structure of Mouse Protocadherin-15 EC4-7

Released:
DOI: 10.2210/pdb5w1d/pdb
PDB entry 5w1d coloured by chain, front view.
PDB entry 5w1d coloured by chain, side view.
PDB entry 5w1d coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural determinants of protocadherin-15 mechanics and function in hearing and balance perception.
Choudhary D, Narui Y, Neel BL, Wimalasena LN, Klanseck CF, De-la-Torre P, Chen C, Araya-Secchi R, Tamilselvan E, Sotomayor M
OpenAccess logo Proc Natl Acad Sci U S A 117 24837-24848 (2020)
PMID: 32963095

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-573178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protocadherin-15 Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 47.12 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99PJ1 (Residues: 401-818; Coverage: 22%)
Gene name: Pcdh15
Sequence domains: Cadherin domain

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 78.863Å b: 78.863Å c: 289.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.279
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Mechanotransduction in mammalian sensory hair cells.
Caprara et al. (2022)
3 more

1 mention without citation

Elasticity of individual protocadherin 15 molecules implicates tip links as the gating springs for hearing.
Bartsch et al. (2019)