› 5w4n

X-ray diffraction
2.2Å resolution

Crystal structure of Streptococcus dysgalactiae SHP pheromone receptor Rgg2(C45S)

Released:
DOI: 10.2210/pdb5w4n/pdb
PDB entry 5w4n coloured by chain, front view.
PDB entry 5w4n coloured by chain, side view.
PDB entry 5w4n coloured by chain, top view.
Source organism: Streptococcus dysgalactiae
Primary publication:
Activating mutations in quorum-sensing regulator Rgg2 and its conformational flexibility in the absence of an intermolecular disulfide bond.
Wilkening RV, Capodagli GC, Khataokar A, Tylor KM, Neiditch MB, Federle MJ
J Biol Chem 292 20544-20557 (2017)
PMID: 29030429

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 33.53 KDa
Source organism: Streptococcus dysgalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0J9X288 (Residues: 1-284; Coverage: 100%)
Gene name: mutR
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand DMS 5 x DMS
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 68.368Å b: 83.268Å c: 129.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.233
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structure-function studies of Rgg binding to pheromones and target promoters reveal a model of transcription factor interplay.
Capodagli et al. (2020)
1 more

1 mention without citation

Activating mutations in quorum-sensing regulator Rgg2 and its conformational flexibility in the absence of an intermolecular disulfide bond.
Wilkening et al. (2017)