› 5w5a

X-ray diffraction
1.85Å resolution

Crystal structure of Mycobacterium tuberculosis CRP-FNR family transcription factor Cmr (Rv1675c)

Released:
DOI: 10.2210/pdb5w5a/pdb
PDB entry 5w5a coloured by chain, front view.
PDB entry 5w5a coloured by chain, side view.
PDB entry 5w5a coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Novel structural features drive DNA binding properties of Cmr, a CRP family protein in TB complex mycobacteria.
Ranganathan S, Cheung J, Cassidy M, Ginter C, Pata JD, McDonough KA
OpenAccess logo Nucleic Acids Res 46 403-420 (2018)
PMID: 29165665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator Cmr Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.38 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMH5 (Residues: 2-244; Coverage: 100%)
Gene names: Rv1675c, cmr
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 51.615Å b: 92.244Å c: 102.758Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Exploiting cAMP signaling in Mycobacterium tuberculosis for drug discovery.
Kathayat et al. (2024)
3 other articles

2 mentions without citation

Novel structural features drive DNA binding properties of Cmr, a CRP family protein in TB complex mycobacteria.
Ranganathan et al. (2018)
1 more