› 5wab

X-ray diffraction
2.45Å resolution

Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase

Released:
DOI: 10.2210/pdb5wab/pdb
PDB entry 5wab coloured by chain, front view.
PDB entry 5wab coloured by chain, side view.
PDB entry 5wab coloured by chain, top view.
Source organism: Bifidobacterium adolescentis ATCC 15703
Primary publication:
Structural and biochemical characterization of a GH3 β-glucosidase from the probiotic bacteria Bifidobacterium adolescentis.
Florindo RN, Souza VP, Manzine LR, Camilo CM, Marana SR, Polikarpov I, Nascimento AS
Biochimie 148 107-115 (2018)
PMID: 29555372

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibronectin type III-like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 751 amino acids
Theoretical weight: 81.19 KDa
Source organism: Bifidobacterium adolescentis ATCC 15703
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: A1A2P2 (Residues: 1-751; Coverage: 100%)
Gene name: BAD_1194
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P1
Unit cell:
a: 94.61Å b: 96.43Å c: 106.77Å
α: 87.76° β: 89.11° γ: 61°
R-values:
R R work R free
0.197 0.195 0.234
Expression system: Escherichia coli str. K-12 substr. DH10B

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Citations

2 review citations

Chemical and nutritional characteristics, and microbial degradation of rapeseed meal recalcitrant carbohydrates: A review.
Long et al. (2022)
1 more

2 mentions without citation

Discovering the Microbial Enzymes Driving Drug Toxicity with Activity-Based Protein Profiling.
Jariwala et al. (2020)
1 more