› 5wlf

X-ray diffraction
1.4Å resolution

Crystal structure of the apo PPS PHD finger

Released:
DOI: 10.2210/pdb5wlf/pdb
PDB entry 5wlf coloured by chain, front view.
PDB entry 5wlf coloured by chain, side view.
PDB entry 5wlf coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
A Unique pH-Dependent Recognition of Methylated Histone H3K4 by PPS and DIDO.
Tencer AH, Gatchalian J, Klein BJ, Khan A, Zhang Y, Strahl BD, van Wely KHM, Kutateladze TG
Structure 25 1530-1539.e3 (2017)
PMID: 28919441

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein partner of snf, isoform A Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.2 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VG78 (Residues: 908-966; Coverage: 3%)
Gene names: CG6525, Dmel\CG6525, Dmel_CG6525, FBgn0082831, PPS, SPP, Spp, pps, spp
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 23.02Å b: 41.06Å c: 26.41Å
α: 90° β: 98.43° γ: 90°
R-values:
R R work R free
0.138 0.136 0.16
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How protons pave the way to aggressive cancers.
Swietach et al. (2023)
1 more

1 mention without citation

A Unique pH-Dependent Recognition of Methylated Histone H3K4 by PPS and DIDO.
Tencer et al. (2017)