5wmm

X-ray diffraction
2.9Å resolution

Crystal structure of an adenylation domain interrupted by a methylation domain (AMA4) from nonribosomal peptide synthetase TioS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-558669 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carrier domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 926 amino acids
Theoretical weight: 102.02 KDa
Source organism: Micromonospora sp. ML1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q333U7 (Residues: 1902-2806; Coverage: 29%)
Gene name: tioS
Sequence domains:
MbtH-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 87 amino acids
Theoretical weight: 9.89 KDa
Source organism: Micromonospora sp. ML1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q333U6 (Residues: 1-71; Coverage: 100%)
Gene name: tioT
Sequence domains: MbtH-like protein
Structure domains: Structural Genomics, Unknown Function 30-nov-00 1gh9 Mol_id

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3221
Unit cell:
a: 136.712Å b: 136.712Å c: 228.234Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.236 0.253
Expression system: Escherichia coli BL21(DE3)