› 5wpi

X-ray diffraction
2.3Å resolution

The virulence-associated protein HsvA from the fire blight pathogen Erwinia amylovora is a polyamine amidinotransferase

Released:
DOI: 10.2210/pdb5wpi/pdb
PDB entry 5wpi coloured by chain, front view.
PDB entry 5wpi coloured by chain, side view.
PDB entry 5wpi coloured by chain, top view.
Source organism: Erwinia amylovora
Primary publication:
The virulence-associated protein HsvA from the fire blight pathogen Erwinia amylovora is a polyamine amidinotransferase.
Shanker S, Schaefer GK, Barnhart BK, Wallace-Kneale VL, Chang D, Coyle TJ, Metzler DA, Huang J, Lawton JA
J Biol Chem 292 21366-21380 (2017)
PMID: 29123034

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HsvA Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 45.12 KDa
Source organism: Erwinia amylovora
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58PW8 (Residues: 1-366; Coverage: 100%)
Gene name: hsvA
Structure domains: L-arginine/glycine Amidinotransferase; Chain A

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P3221
Unit cell:
a: 127.543Å b: 127.543Å c: 92.229Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.226
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Substrate Promiscuity of a Paralytic Shellfish Toxin Amidinotransferase.
Lukowski et al. (2020)

1 mention without citation

The virulence-associated protein HsvA from the fire blight pathogen Erwinia amylovora is a polyamine amidinotransferase.
Shanker et al. (2017)