› 5ws4

X-ray diffraction
3.4Å resolution

Crystal structure of tripartite-type ABC transporter MacB from Acinetobacter baumannii

Released:
DOI: 10.2210/pdb5ws4/pdb
PDB entry 5ws4 coloured by chain, front view.
PDB entry 5ws4 coloured by chain, side view.
PDB entry 5ws4 coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Crystal structure of tripartite-type ABC transporter MacB from Acinetobacter baumannii.
Okada U, Yamashita E, Neuberger A, Morimoto M, van Veen HW, Murakami S
OpenAccess logo Nat Commun 8 1336 (2017)
PMID: 29109439

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 671 amino acids
Theoretical weight: 72.27 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0D8G707 (Residues: 1-664; Coverage: 100%)
Gene names: ABR2091_0527, ABUW_3363, AUO97_10105, B9X95_00195, CV954_017100, DOL94_08180, EA722_00035, EKS29_09795, EWP49_09045, F2P40_14105, F4T85_01915, FJU42_00095, FPK81_09035, FR761_03910, GNY86_09180, GSE42_02490, H0529_13670, IAG11_00035, IHV20_04620, IMO23_02640, LV35_03249, NCTC13305_01910, OMP06_02860, SAMEA104305318_00088, macB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand AT4 2 x AT4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P41212
Unit cell:
a: 229.652Å b: 229.652Å c: 154.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
11 more

8 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
7 more