› 5wt6

X-ray diffraction
3.3Å resolution

Cysteine persulfide intermediate of NifS from Helicobacter pylori

Released:
DOI: 10.2210/pdb5wt6/pdb
PDB entry 5wt6 coloured by chain, front view.
PDB entry 5wt6 coloured by chain, side view.
PDB entry 5wt6 coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Entry authors: Fujishiro T, Nakamura R, Takahashi Y

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase IscS Chain: A
Molecule details ›
Chain: A
Length: 401 amino acids
Theoretical weight: 44.16 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: O25008 (Residues: 1-387; Coverage: 100%)
Gene names: HP_0220, iscS
Sequence domains: Aminotransferase class-V

Ligands and Environments

3 bound ligands:
Ligand IPA 1 x IPA
Ligand CL 1 x CL
Ligand PDA 1 x PDA
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P41212
Unit cell:
a: 102.75Å b: 102.75Å c: 131.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.245
Expression system: Escherichia coli

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