› 5wvy

X-ray diffraction
2Å resolution

The crystal structure of Cren7 mutant L28V in complex with dsDNA

Released:
DOI: 10.2210/pdb5wvy/pdb
PDB entry 5wvy coloured by chain, front view.
PDB entry 5wvy coloured by chain, side view.
PDB entry 5wvy coloured by chain, top view.
Source organisms:
Primary publication:
Roles of Leu28 side chain intercalation in the interaction between Cren7 and DNA.
Zhang Z, Zhao M, Wang L, Chen Y, Dong Y, Gong Y, Huang L
Biochem J 474 1727-1739 (2017)
PMID: 28377493

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119364 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Chromatin protein Cren7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 6.66 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZE3 (Residues: 1-60; Coverage: 100%)
Gene names: SSO6901, creN7
Sequence domains: Chromatin protein Cren7
Structure domains: Chromatin protein Cren7
DNA (5'-D(*GP*TP*GP*AP*TP*CP*AP*C)-3') Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 78.884Å b: 78.867Å c: 104.218Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.236
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Archaea: The Final Frontier of Chromatin.
Laursen et al. (2021)
3 other articles