› 5wys

X-ray diffraction
3Å resolution

luciferase with inhibitor 3i

Released:
DOI: 10.2210/pdb5wys/pdb
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PDB entry 5wys coloured by chain, front view.
PDB entry 5wys coloured by chain, side view.
PDB entry 5wys coloured by chain, top view.
Source organism: Photinus pyralis
Primary publication:
Inhibiting Firefly Bioluminescence by Chalcones.
Zhang H, Su J, Lin Y, Bai H, Liu J, Chen H, Du L, Gu L, Li M
Anal Chem 89 6099-6105 (2017)
PMID: 28492074

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 60.82 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 7V6 1 x 7V6
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MACSCIENCE
Spacegroup: P41212
Unit cell:
a: 118.141Å b: 118.141Å c: 96.194Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.195 0.27
Expression system: Escherichia coli BL21

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Citations

1 review citation

The elusive relationship between structure and colour emission in beetle luciferases.
Carrasco-López et al. (2021)
3 other articles

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