› 5x2j

X-ray diffraction
1.4Å resolution

Crystal structure of a recombinant hybrid manganese superoxide dismutase from Staphylococcus equorum and Staphylococcus saprophyticus

Released:
DOI: 10.2210/pdb5x2j/pdb
PDB entry 5x2j coloured by chain, front view.
PDB entry 5x2j coloured by chain, side view.
PDB entry 5x2j coloured by chain, top view.
Source organism: Staphylococcus equorum
Primary publication:
The first crystal structure of manganese superoxide dismutase from the genus Staphylococcus.
Retnoningrum DS, Yoshida H, Arumsari S, Kamitori S, Ismaya WT
Acta Crystallogr F Struct Biol Commun 74 135-142 (2018)
PMID: 29497016

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.67 KDa
Source organism: Staphylococcus equorum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1E5TT85 (Residues: 1-199; Coverage: 100%)
Gene name: M4L21_03170
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3221
Unit cell:
a: 57.362Å b: 57.362Å c: 105.762Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.144 0.142 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Biochemical Properties of Manganese in Plants.
Schmidt et al. (2019)
1 more

1 mention without citation

The first crystal structure of manganese superoxide dismutase from the genus Staphylococcus.
Retnoningrum et al. (2018)