› 5x45

X-ray diffraction
2.6Å resolution

Crystal structure of 2A protease from Human rhinovirus C15

Released:
DOI: 10.2210/pdb5x45/pdb
PDB entry 5x45 coloured by chain, front view.
PDB entry 5x45 coloured by chain, side view.
PDB entry 5x45 coloured by chain, top view.
Source organism: Rhinovirus C
Primary publication:
Structural view of the 2A protease from human rhinovirus C15.
Ling H, Yang P, Hou H, Sun Y
Acta Crystallogr F Struct Biol Commun 74 255-261 (2018)
PMID: 29633974

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease 2A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 15.24 KDa
Source organism: Rhinovirus C
Expression system: Escherichia coli
UniProt:
  • Canonical: E5D8F2 (Residues: 847-983; Coverage: 6%)
Sequence domains: Picornavirus core protein 2A
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2221
Unit cell:
a: 80.992Å b: 85.37Å c: 208.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.215
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structural view of the 2A protease from human rhinovirus C15.
Ling et al. (2018)