› 5x5u

X-ray diffraction
2.3Å resolution

Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (KGSADH) complexed with NAD

Released:
DOI: 10.2210/pdb5x5u/pdb
PDB entry 5x5u coloured by chain, front view.
PDB entry 5x5u coloured by chain, side view.
PDB entry 5x5u coloured by chain, top view.
Source organism: Azospirillum brasilense
Primary publication:
Structural insights into the production of 3-hydroxypropionic acid by aldehyde dehydrogenase from Azospirillum brasilense.
Son HF, Park S, Yoo TH, Jung GY, Kim KJ
OpenAccess logo Sci Rep 7 46005 (2017)
PMID: 28393833

Function and Biology Details

Reactions catalysed: 
Succinate semialdehyde + NAD(+) + H(2)O = succinate + NADH
2,5-dioxopentanoate + NADP(+) + H(2)O = 2-oxoglutarate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutaric semialdehyde dehydrogenase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 53.86 KDa
Source organism: Azospirillum brasilense
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1JUP4 (Residues: 2-481; Coverage: 100%)
Gene name: araE
Sequence domains: Aldehyde dehydrogenase family

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P4322
Unit cell:
a: 129.305Å b: 129.305Å c: 118.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria.
Jers et al. (2019)
4 other articles

6 mentions without citation

A Biochemical Perspective of the Nonstructural Proteins (NSPs) and the Spike Protein of SARS CoV-2.
Yoshimoto FK. (2021)
5 more