› 5x5x

X-ray diffraction
1.9Å resolution

Crystal structure of the Fab fragment of anti-osteocalcin C-terminal peptide antibody KTM219

Released:
DOI: 10.2210/pdb5x5x/pdb
PDB entry 5x5x coloured by chain, front view.
PDB entry 5x5x coloured by chain, side view.
PDB entry 5x5x coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Komatsu M, Dong J, Ueda H, Arai R

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213945 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fd chain of anti-osteocalcin antibody KTM219 Chain: H
Molecule details ›
Chain: H
Length: 249 amino acids
Theoretical weight: 26.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
L chain of anti-osteocalcin antibody KTM219 Chain: L
Molecule details ›
Chain: L
Length: 232 amino acids
Theoretical weight: 25.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand PEG 4 x PEG
Ligand PG4 2 x PG4
Ligand FMT 3 x FMT
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Unit cell:
a: 64.8Å b: 71.472Å c: 96.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.224
Expression system: Escherichia coli

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