5x5y

X-ray diffraction
3.46Å resolution

A membrane protein complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-574800 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lipopolysaccharide export system ATP-binding protein LptB Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.32 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HVV6 (Residues: 1-241; Coverage: 100%)
Gene name: PA4461
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
LPS export ABC transporter permease LptG Chain: G
Molecule details ›
Chain: G
Length: 355 amino acids
Theoretical weight: 39.24 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HXH5 (Residues: 1-355; Coverage: 100%)
Gene name: PA3827
Sequence domains: Lipopolysaccharide export system permease LptF/LptG
Lipopolysaccharide export system permease protein LptF Chain: F
Molecule details ›
Chain: F
Length: 362 amino acids
Theoretical weight: 40.34 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HXH4 (Residues: 1-362; Coverage: 97%)
Gene name: PA3828
Sequence domains: Lipopolysaccharide export system permease LptF/LptG

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 69.952Å b: 159.868Å c: 166.786Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.269 0.304
Expression system: Escherichia coli BL21(DE3)