› 5x6h

X-ray diffraction
3.1Å resolution

Crystal Structure of SMAD5-MH1/GC-BRE DNA complex

Released:
DOI: 10.2210/pdb5x6h/pdb
PDB entry 5x6h coloured by chain, front view.
PDB entry 5x6h coloured by chain, side view.
PDB entry 5x6h coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the Smad5 MH1 domain to recognize different DNA sequences.
Chai N, Li WX, Wang J, Wang ZX, Yang SM, Wu JW
OpenAccess logo Nucleic Acids Res 43 9051-64 (2015)
PMID: 26304548

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-509997 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 5 Chain: B
Molecule details ›
Chain: B
Length: 150 amino acids
Theoretical weight: 17.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P97454 (Residues: 1-143; Coverage: 31%)
Gene names: Madh5, Msmad5, Smad5
Sequence domains: MH1 domain
Structure domains: SMAD MH1 domain
DNA (5'-D(P*GP*TP*AP*TP*GP*GP*CP*GP*CP*CP*AP*TP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6422
Unit cell:
a: 92.867Å b: 92.867Å c: 83.709Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Specification of BMP Signaling.
Nickel et al. (2019)
5 more

1 mention without citation

Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes.
Ruiz et al. (2021)