› 5xa7

X-ray diffraction
3.2Å resolution

Complete structure factors and an atomic model of the calcium pump (SERCA1A) and associated phospholipids in the E1-2CA2+ crystals

Released:
DOI: 10.2210/pdb5xa7/pdb
PDB entry 5xa7 coloured by chain, front view.
PDB entry 5xa7 coloured by chain, side view.
PDB entry 5xa7 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Protein-phospholipid interplay revealed with crystals of a calcium pump.
Norimatsu Y, Hasegawa K, Shimizu N, Toyoshima C
Nature 545 193-198 (2017)
PMID: 28467821

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: A
Molecule details ›
Chain: A
Length: 995 amino acids
Theoretical weight: 109.63 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 1-993; Coverage: 99%)
  • Best match: P04191-2 (Residues: 1-994)
Gene name: ATP2A1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand NA 1 x NA
Ligand PCW 34 x PCW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 166.2Å b: 64.545Å c: 146.22Å
α: 90° β: 98.12° γ: 90°
R-values:
R R work R free
0.226 0.225 0.244

Quick links

Citations

23 review citations

Understanding the diversity of membrane lipid composition.
Harayama et al. (2018)
22 more

4 mentions without citation

Essay on Biomembrane Structure.
Gerle C. (2019)
3 more