› 5xe2

X-ray diffraction
2.01Å resolution

Endoribonuclease from Mycobacterial species

Released:
DOI: 10.2210/pdb5xe2/pdb
PDB entry 5xe2 coloured by chain, front view.
PDB entry 5xe2 coloured by chain, side view.
PDB entry 5xe2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural analyses of the MazEF4 toxin-antitoxin pair in Mycobacterium tuberculosis provide evidence for a unique extracellular death factor.
Ahn DH, Lee KY, Lee SJ, Park SJ, Yoon HJ, Kim SJ, Lee BJ
J Biol Chem 292 18832-18847 (2017)
PMID: 28972145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease MazF4 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WII5 (Residues: 1-105; Coverage: 100%)
Gene names: MTCY277.17, Rv1495, mazF-mt7, mazF4
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2221
Unit cell:
a: 49.475Å b: 65.548Å c: 57.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Variety in the Common Theme of Translation Inhibition by Type II Toxin-Antitoxin Systems.
Jurėnas et al. (2020)
1 more

5 mentions without citation

Jumbo Phages: A Comparative Genomic Overview of Core Functions and Adaptions for Biological Conflicts.
M Iyer et al. (2021)
4 more