› 5xhi

X-ray diffraction
1.26Å resolution

Crystal structure of Frog M-ferritin D38A mutant

Released:
DOI: 10.2210/pdb5xhi/pdb
PDB entry 5xhi coloured by chain, front view.
PDB entry 5xhi coloured by chain, side view.
PDB entry 5xhi coloured by chain, top view.
Source organism: Lithobates catesbeianus
Primary publication:
Surface charge dependent separation of modified and hybrid ferritin in native PAGE: Impact of lysine 104.
Subhadarshanee B, Mohanty A, Jagdev MK, Vasudevan D, Behera RK
Biochim Biophys Acta Proteins Proteom 1865 1267-1273 (2017)
PMID: 28739445

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-530297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin, middle subunit Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.36 KDa
Source organism: Lithobates catesbeianus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07798 (Residues: 2-175; Coverage: 99%)
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand MG 11 x MG
Ligand CL 27 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: F432
Unit cell:
a: 184.09Å b: 184.09Å c: 184.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.127 0.125 0.153
Expression system: Escherichia coli

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Citations

1 review citation

Ferritin: A Promising Nanoreactor and Nanocarrier for Bionanotechnology.
Mohanty et al. (2022)
5 other articles