› 5xib

X-ray diffraction
2.3Å resolution

Crystal Structure of HasAp with Fe-5,15-Diphenylporphyrin

Released:
DOI: 10.2210/pdb5xib/pdb
PDB entry 5xib coloured by chain, front view.
PDB entry 5xib coloured by chain, side view.
PDB entry 5xib coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structures of the Heme Acquisition Protein HasA with Iron(III)-5,15-Diphenylporphyrin and Derivatives Thereof as an Artificial Prosthetic Group.
Uehara H, Shisaka Y, Nishimura T, Sugimoto H, Shiro Y, Miyake Y, Shinokubo H, Watanabe Y, Shoji O
Angew Chem Int Ed Engl 56 15279-15283 (2017)
PMID: 28921809

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme acquisition protein HasAp Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 18.9 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XD33 (Residues: 1-184; Coverage: 90%)
Gene names: PA3407, hasAp
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

1 bound ligand:
Ligand WUP 2 x WUP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P61
Unit cell:
a: 154.551Å b: 154.551Å c: 36.793Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.224
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics.
Qin et al. (2022)
2 more