› 5xn9

X-ray diffraction
1.45Å resolution

Crystal structure of a secretary abundant heat soluble (SAHS) protein from Ramazzottius varieornatus (from monomer sample)

Released:
DOI: 10.2210/pdb5xn9/pdb
PDB entry 5xn9 coloured by chain, front view.
PDB entry 5xn9 coloured by chain, side view.
PDB entry 5xn9 coloured by chain, top view.
Source organism: Ramazzottius varieornatus
Primary publication:
Structural insights into a secretory abundant heat-soluble protein from an anhydrobiotic tardigrade, Ramazzottius varieornatus.
Fukuda Y, Miura Y, Mizohata E, Inoue T
FEBS Lett 591 2458-2469 (2017)
PMID: 28703282

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Secretory-abundant heat soluble protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 16.19 KDa
Source organism: Ramazzottius varieornatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J7MFT5 (Residues: 31-167; Coverage: 91%)
Gene names: RvY_02423, SAHS1

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand ZN 4 x ZN
Ligand CL 2 x CL
Ligand EDO 13 x EDO
Ligand ACY 3 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 53.866Å b: 57.505Å c: 111.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The biology of tardigrade disordered proteins in extreme stress tolerance.
Hesgrove et al. (2020)
1 more

1 mention without citation

Evidence that immunization with TP0751, a bipartite Treponema pallidum lipoprotein with an intrinsically disordered region and lipocalin fold, fails to protect in the rabbit model of experimental syphilis.
Luthra et al. (2020)