5xu1 › Citations

Structure of a MacAB-like efflux pump from Streptococcus pneumoniae.

Fig. 1
Fig. 2
Fig. 3
Yang HB, Hou WT, Cheng MT, Jiang YL, Chen Y, Zhou CZ
OpenAccess logo Nat Commun 9 196 (2018)
Cited: 22 times
EuropePMC logo PMID: 29335499

Abstract

The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694-0695 at 3.3 Å and Spr0693 at 3.0 Å resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694-0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria.

Reviews - 5xu1 mentioned but not cited (5)

  1. Structural and functional diversity calls for a new classification of ABC transporters. Thomas C, Aller SG, Beis K, Carpenter EP, Chang G, Chen L, Dassa E, Dean M, Duong Van Hoa F, Ekiert D, Ford R, Gaudet R, Gong X, Holland IB, Huang Y, Kahne DK, Kato H, Koronakis V, Koth CM, Lee Y, Lewinson O, Lill R, Martinoia E, Murakami S, Pinkett HW, Poolman B, Rosenbaum D, Sarkadi B, Schmitt L, Schneider E, Shi Y, Shyng SL, Slotboom DJ, Tajkhorshid E, Tieleman DP, Ueda K, Váradi A, Wen PC, Yan N, Zhang P, Zheng H, Zimmer J, Tampé R. FEBS Lett 594 3767-3775 (2020)
  2. Antibiotic Resistance Mediated by the MacB ABC Transporter Family: A Structural and Functional Perspective. Greene NP, Kaplan E, Crow A, Koronakis V. Front Microbiol 9 950 (2018)
  3. Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria. Alav I, Kobylka J, Kuth MS, Pos KM, Picard M, Blair JMA, Bavro VN. Chem Rev 121 5479-5596 (2021)
  4. Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases. Kim J, Cater RJ, Choy BC, Mancia F. J Mol Biol 433 167005 (2021)
  5. The ATP-Binding Cassette (ABC) Transport Systems in Mycobacterium tuberculosis: Structure, Function, and Possible Targets for Therapeutics. Cassio Barreto de Oliveira M, Balan A. Biology (Basel) 9 E443 (2020)

Articles - 5xu1 mentioned but not cited (3)



Reviews citing this publication (4)

  1. Resistance Mechanisms to Antimicrobial Peptides in Gram-Positive Bacteria. Assoni L, Milani B, Carvalho MR, Nepomuceno LN, Waz NT, Guerra MES, Converso TR, Darrieux M. Front Microbiol 11 593215 (2020)
  2. Alternatives to Conventional Antibiotic Therapy: Potential Therapeutic Strategies of Combating Antimicrobial-Resistance and Biofilm-Related Infections. Xu Q, Hu X, Wang Y. Mol Biotechnol 63 1103-1124 (2021)
  3. Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system. Jo I, Kim JS, Xu Y, Hyun J, Lee K, Ha NC. J Microbiol 57 185-194 (2019)
  4. ABC Transporters in Bacterial Nanomachineries. Bilsing FL, Anlauf MT, Hachani E, Khosa S, Schmitt L. Int J Mol Sci 24 6227 (2023)

Articles citing this publication (10)



Quick links