› 5xu6

X-ray diffraction
2.35Å resolution

Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPK1) from Cryptococcus neoformans

Released:
DOI: 10.2210/pdb5xu6/pdb
PDB entry 5xu6 coloured by chain, front view.
PDB entry 5xu6 coloured by chain, side view.
PDB entry 5xu6 coloured by chain, top view.
Source organism: Cryptococcus neoformans var. grubii H99
Primary publication:
Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase from Cryptococcus neoformans.
Oh J, Lee DG, Bahn YS, Rhee S
J Struct Biol 200 118-123 (2017)
PMID: 28919350

Function and Biology Details

Reaction catalysed: 
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-pentakisphosphate 2-kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 417 amino acids
Theoretical weight: 46.8 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J9VKS8 (Residues: 1-415; Coverage: 100%)
Gene name: CNAG_01294
Sequence domains: Inositol-pentakisphosphate 2-kinase

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Unit cell:
a: 95.81Å b: 59.74Å c: 379.674Å
α: 90° β: 96.36° γ: 90°
R-values:
R R work R free
0.236 0.234 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Fungal Kinases With a Sweet Tooth: Pleiotropic Roles of Their Phosphorylated Inositol Sugar Products in the Pathogenicity of Cryptococcus neoformans Present Novel Drug Targeting Opportunities.
Lev et al. (2019)