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Electron cryo-microscopy structure of LbuCas13a-crRNA binary complex

Electron Microscopy
3.2Å resolution
PDB entry 5xwy coloured by chain, front view.
PDB entry 5xwy coloured by chain, side view.
PDB entry 5xwy coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CRISPR-associated endoribonuclease Cas13a and RNA
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Multimeric state: hetero dimer
Accessible surface area: 57677.52 Å2
Buried surface area: 11393.62 Å2
Dissociation area: 5,696.81 Å2
Dissociation energy (ΔGdiss): 104.68 kcal/mol
Dissociation entropy (TΔSdiss): 14.7 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-111733

Macromolecules

CRISPR-associated endoribonuclease Cas13a
Chain: A
Length: 1159 amino acids
Theoretical weight: 138.56 KDa
Source organism: Leptotrichia buccalis C-1013-b
Expression system: Escherichia coli
UniProt:
  • Canonical: C7NBY4 (Residues: 1-1159; Coverage: 100%)
Gene names: Lebu_1799, c2c2, cas13a
CRISPR-associated endoribonuclease Cas13a in PDB entry 5xwy, assembly 1, front view.
CRISPR-associated endoribonuclease Cas13a in PDB entry 5xwy, assembly 1, side view.
CRISPR-associated endoribonuclease Cas13a in PDB entry 5xwy, assembly 1, top view.
PDBe-KB: UniProt Coverage View: C7NBY4  
111591002003004005006007008009001,0001,100
 
5001000
UniProt
C7NBY4
Chains
Secondary structure
Flexibility predictions
Early folding residue predictions
Sequence conservation
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RNA chain B
Name: RNA (59-MER)
Representative chains: B
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 59 nucleotides
Theoretical weight: 18.87 KDa
RNA (59-MER) in PDB entry 5xwy, assembly 1, front view.
RNA (59-MER) in PDB entry 5xwy, assembly 1, side view.
RNA (59-MER) in PDB entry 5xwy, assembly 1, top view.
159510152025303540455055
 
204060GGACCACCCCAAAAAUGAAGGGGACUAAAACACAAAUCUAUCUGAAUAAACUCUUCUUC
Chains
Chain B (auth B)
Interaction interfaces
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