› 5xyv

X-ray diffraction
2.1Å resolution

Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain

Released:
DOI: 10.2210/pdb5xyv/pdb
PDB entry 5xyv coloured by chain, front view.
PDB entry 5xyv coloured by chain, side view.
PDB entry 5xyv coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.
Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y
EMBO Rep 19 (2018)
PMID: 29858487

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181524 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromo domain-containing protein rhino Chains: A, B
Molecule details ›
Chains: A, B
Length: 78 amino acids
Theoretical weight: 8.58 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7JXA8 (Residues: 353-418; Coverage: 16%)
Gene names: CG10683, HP1, HP1D, rhi, rhino
Protein deadlock Chains: C, D
Molecule details ›
Chains: C, D
Length: 60 amino acids
Theoretical weight: 7.11 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VIF5 (Residues: 1-60; Coverage: 6%)
Gene names: CG9252, del

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 47.752Å b: 57.097Å c: 101.002Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.213 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

piRNA-Guided Genome Defense: From Biogenesis to Silencing.
Czech et al. (2018)
3 more

1 mention without citation

Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.
Yu et al. (2018)