› 5xze

X-ray diffraction
2.18Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119417 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 42.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8C6L5 (Residues: 147-507; Coverage: 71%)
Gene names: Cgas, Mb21d1
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*AP*TP*TP*GP*CP*CP*GP*AP*AP*GP*AP*CP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.63 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(P*CP*GP*TP*CP*TP*TP*CP*GP*GP*CP*AP*AP*TP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand AE7 1 x AE7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I222
Unit cell:
a: 85.285Å b: 98.633Å c: 129.175Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.281
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

64 review citations

DAMP-sensing receptors in sterile inflammation and inflammatory diseases.
Gong et al. (2020)
63 more

3 mentions without citation

The World of Cyclic Dinucleotides in Bacterial Behavior.
Aline Dias da et al. (2020)
2 more