› 5xzj

X-ray diffraction
1.98Å resolution

Crystal structure of the Zn-directed tetramer of the engineered cyt cb562 variant, C96T/AB5

Released:
DOI: 10.2210/pdb5xzj/pdb
PDB entry 5xzj coloured by chain, front view.
PDB entry 5xzj coloured by chain, side view.
PDB entry 5xzj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Importance of Scaffold Flexibility/Rigidity in the Design and Directed Evolution of Artificial Metallo-β-lactamases.
Song WJ, Yu J, Tezcan FA
J Am Chem Soc 139 16772-16779 (2017)
PMID: 28992705

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

3 bound ligands:
Ligand HEC 4 x HEC
Ligand ZN 14 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 60.747Å b: 63.481Å c: 127.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.217
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
6 more

1 mention without citation

Symmetry-related residues as promising hotspots for the evolution of de novo oligomeric enzymes.
Yu et al. (2021)