5y0p Citations

Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis.

Nat Chem Biol 14 1010-1020 (2018)
Related entries: 5y0n, 5y0o, 5y0q, 5y0r, 5y0s, 5y0t

Cited: 27 times
EuropePMC logo PMID: 30150682

Abstract

Modification of tRNA anticodons plays a critical role in ensuring accurate translation. N4-acetylcytidine (ac4C) is present at the anticodon first position (position 34) of bacterial elongator tRNAMet. Herein, we identified Bacillus subtilis ylbM (renamed tmcAL) as a novel gene responsible for ac4C34 formation. Unlike general acetyltransferases that use acetyl-CoA, TmcAL activates an acetate ion to form acetyladenylate and then catalyzes ac4C34 formation through a mechanism similar to tRNA aminoacylation. The crystal structure of TmcAL with an ATP analog reveals the molecular basis of ac4C34 formation. The ΔtmcAL strain displayed a cold-sensitive phenotype and a strong genetic interaction with tilS that encodes the enzyme responsible for synthesizing lysidine (L) at position 34 of tRNAIle to facilitate AUA decoding. Mistranslation of the AUA codon as Met in the ΔtmcAL strain upon tilS repression suggests that ac4C34 modification of tRNAMet and L34 modification of tRNAIle act cooperatively to prevent misdecoding of the AUA codon.

Reviews citing this publication (10)

  1. Functions of Bacterial tRNA Modifications: From Ubiquity to Diversity. de Crécy-Lagard V, Jaroch M. Trends Microbiol 29 41-53 (2021)
  2. Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions. Zhang J. Wiley Interdiscip Rev RNA 11 e1600 (2020)
  3. Critical Roles of N6-Methyladenosine (m6A) in Cancer and Virus Infection. Asada K, Bolatkan A, Takasawa K, Komatsu M, Kaneko S, Hamamoto R. Biomolecules 10 E1071 (2020)
  4. Mechanisms of epitranscriptomic gene regulation. Seo KW, Kleiner RE. Biopolymers 112 e23403 (2021)
  5. Probing the diversity and regulation of tRNA modifications. Kimura S, Srisuknimit V, Waldor MK. Curr Opin Microbiol 57 41-48 (2020)
  6. Making Sense of "Nonsense" and More: Challenges and Opportunities in the Genetic Code Expansion, in the World of tRNA Modifications. Lateef OM, Akintubosun MO, Olaoba OT, Samson SO, Adamczyk M. Int J Mol Sci 23 938 (2022)
  7. From canonical to modified nucleotides: balancing translation and metabolism. Accornero F, Ross RL, Alfonzo JD. Crit Rev Biochem Mol Biol 55 525-540 (2020)
  8. Interplay between Host tRNAs and HIV-1: A Structural Perspective. Zhang J. Viruses 13 1819 (2021)
  9. Regulation of the epigenome through RNA modifications. Patrasso EA, Raikundalia S, Arango D. Chromosoma 132 231-246 (2023)
  10. The Long Road to a Synthetic Self-Replicating Central Dogma. De Capitani J, Mutschler H. Biochemistry 62 1221-1232 (2023)

Articles citing this publication (17)