› 5y24

X-ray diffraction
1.92Å resolution

Crystal structure of AimR from Bacillus phage SPbeta in complex with its signalling peptide

Released:
DOI: 10.2210/pdb5y24/pdb
PDB entry 5y24 coloured by chain, front view.
PDB entry 5y24 coloured by chain, side view.
PDB entry 5y24 coloured by chain, top view.
Source organism: Spbetavirus SPbeta
Primary publication:
Structural basis of the arbitrium peptide-AimR communication system in the phage lysis-lysogeny decision.
Wang Q, Guan Z, Pei K, Wang J, Liu Z, Yin P, Peng D, Zou T
Nat Microbiol 3 1266-1273 (2018)
PMID: 30224798

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130262 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AimR transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 47.42 KDa
Source organism: Spbetavirus SPbeta
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O64094 (Residues: 1-386; Coverage: 100%)
Gene names: aimR, yopK
Sequence domains: AimR transcriptional regulator-like
GLY-MET-PRO-ARG-GLY-ALA Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 589 Da
Source organism: Spbetavirus SPbeta
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand BR 5 x BR
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Unit cell:
a: 33.55Å b: 119.605Å c: 214.359Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.249
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Phages against Pathogenic Bacterial Biofilms and Biofilm-Based Infections: A Review.
Liu et al. (2022)
3 more