› 5y2j

X-ray diffraction
2.55Å resolution

Crystal structure of the oligomerization domain of NSP4 from rotavirus strain MF66

Released:
DOI: 10.2210/pdb5y2j/pdb
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PDB entry 5y2j coloured by chain, front view.
PDB entry 5y2j coloured by chain, side view.
PDB entry 5y2j coloured by chain, top view.
Source organism: Bovine rotavirus G10
Primary publication:
New tetrameric forms of the rotavirus NSP4 with antiparallel helices.
Kumar S, Ramappa R, Pamidimukkala K, Rao CD, Suguna K
Arch Virol 163 1531-1547 (2018)
PMID: 29455326

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural glycoprotein 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 53 amino acids
Theoretical weight: 6.43 KDa
Source organism: Bovine rotavirus G10
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6QT01 (Residues: 95-146; Coverage: 30%)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 53.61Å b: 69.57Å c: 69.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.258
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

A Structural Analysis of Proteinaceous Nanotube Cavities and Their Applications in Nanotechnology.
Heide et al. (2022)
4 other articles

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