5y5a

X-ray diffraction
2.21Å resolution

Crystal structure of Est1 and Cdc13

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-563642 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nonsense-mediated mRNA decay factor Chain: A
Molecule details ›
Chain: A
Length: 567 amino acids
Theoretical weight: 66.37 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6CJ80 (Residues: 2-600; Coverage: 63%)
Gene name: KLLA0_F20702g
Sequence domains:
Telomeric single stranded DNA binding POT1/Cdc13 domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 26 amino acids
Theoretical weight: 3.03 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6CJ70 (Residues: 213-238; Coverage: 3%)
Gene name: KLLA0_F20922g

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1, SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 108.611Å b: 41.726Å c: 147.06Å
α: 90° β: 97.45° γ: 90°
R-values:
R R work R free
0.178 0.176 0.224
Expression system: Escherichia coli BL21(DE3)