› 5y89

X-ray diffraction
2.4Å resolution

Periplasmic heme-binding protein BhuT in complex with one heme (holo-1)

Released:
DOI: 10.2210/pdb5y89/pdb
PDB entry 5y89 coloured by chain, front view.
PDB entry 5y89 coloured by chain, side view.
PDB entry 5y89 coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Primary publication:
Structural basis for binding and transfer of heme in bacterial heme-acquisition systems.
Naoe Y, Nakamura N, Rahman MM, Tosha T, Nagatoishi S, Tsumoto K, Shiro Y, Sugimoto H
Proteins 85 2217-2230 (2017)
PMID: 28913898

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 28.02 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EKB3 (Residues: 40-305; Coverage: 87%)
Gene names: BCAM2628, hmuT
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand ACT 2 x ACT
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P6122
Unit cell:
a: 199.166Å b: 199.166Å c: 117.736Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.2 0.22
Expression system: Escherichia coli

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Citations

1 review citation

Heme Uptake and Utilization by Gram-Negative Bacterial Pathogens.
Richard et al. (2019)
1 other article