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Structural basis of host recognition and biofilm formation by Salmonella Saf pili.

<div class='caption-title'>Recombinant expression strategy of SafD-dsc and SafDAA-dsc.</div>
<div class='caption-title'>Sequence alignment of SafD (A) and SafA (B) in Salmonella enterica strains.</div>
<div class='caption-title'>Crystal structure of SafD-dsc.</div>
Zeng L, Zhang L, Wang P, Meng G
OpenAccess logo Elife 6 (2017)
Cited: 13 times
EuropePMC logo PMID: 29125121

Abstract

Pili are critical in host recognition, colonization and biofilm formation during bacterial infection. Here, we report the crystal structures of SafD-dsc and SafD-SafA-SafA (SafDAA-dsc) in Saf pili. Cell adherence assays show that SafD and SafA are both required for host recognition, suggesting a poly-adhesive mechanism for Saf pili. Moreover, the SafDAA-dsc structure, as well as SAXS characterization, reveals an unexpected inter-molecular oligomerization, prompting the investigation of Saf-driven self-association in biofilm formation. The bead/cell aggregation and biofilm formation assays are used to demonstrate the novel function of Saf pili. Structure-based mutants targeting the inter-molecular hydrogen bonds and complementary architecture/surfaces in SafDAA-dsc dimers significantly impaired the Saf self-association activity and biofilm formation. In summary, our results identify two novel functions of Saf pili: the poly-adhesive and self-associating activities. More importantly, Saf-Saf structures and functional characterizations help to define a pili-mediated inter-cellular oligomerizaiton mechanism for bacterial aggregation, colonization and ultimate biofilm formation.

Reviews citing this publication (2)

  1. Mechanisms and Impact of Biofilms and Targeting of Biofilms Using Bioactive Compounds-A Review. Samrot AV, Abubakar Mohamed A, Faradjeva E, Si Jie L, Hooi Sze C, Arif A, Chuan Sean T, Norbert Michael E, Yeok Mun C, Xiao Qi N, Ling Mok P, Kumar SS. Medicina (Kaunas) 57 839 (2021)
  2. Bacterial biofilms in the human body: prevalence and impacts on health and disease. Perry EK, Tan MW. Front Cell Infect Microbiol 13 1237164 (2023)

Articles citing this publication (11)

  1. Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference to Helicobacter pylori Infection. Rizzato C, Torres J, Kasamatsu E, Camorlinga-Ponce M, Bravo MM, Canzian F, Kato I. Front Microbiol 10 846 (2019)
  2. Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg. Antony L, Behr M, Sockett D, Miskimins D, Aulik N, Christopher-Hennings J, Nelson E, Allard MW, Scaria J. Gut Pathog 10 53 (2018)
  3. Role of CpxR in Biofilm Development: Expression of Key Fimbrial, O-Antigen and Virulence Operons of Salmonella Enteritidis. Shetty D, Abrahante JE, Chekabab SM, Wu X, Korber DR, Vidovic S. Int J Mol Sci 20 E5146 (2019)
  4. Targeting effect of berberine on type I fimbriae of Salmonella Typhimurium and its effective inhibition of biofilm. Xu C, Wang F, Huang F, Yang M, He D, Deng L. Appl Microbiol Biotechnol 105 1563-1573 (2021)
  5. Genomic comparison of diverse Salmonella serovars isolated from swine. Gupta SK, Sharma P, McMillan EA, Jackson CR, Hiott LM, Woodley T, Humayoun SB, Barrett JB, Frye JG, McClelland M. PLoS One 14 e0224518 (2019)
  6. Factors Required for Adhesion of Salmonella enterica Serovar Typhimurium to Corn Salad (Valerianella locusta). Elpers L, Kretzschmar J, Nuccio SP, Bäumler AJ, Hensel M. Appl Environ Microbiol 86 e02757-19 (2020)
  7. Electron cryo-microscopy reveals the structure of the archaeal thread filament. Gaines MC, Isupov MN, Sivabalasarma S, Haque RU, McLaren M, Mollat CL, Tripp P, Neuhaus A, Gold VAM, Albers SV, Daum B. Nat Commun 13 7411 (2022)
  8. E. coli Common pili promote the fitness and virulence of a hybrid aEPEC/ExPEC strain within diverse host environments. Munhoz DD, Richards AC, Santos FF, Mulvey MA, Piazza RMF. Gut Microbes 15 2190308 (2023)
  9. A Potential Adhesin/Invasin STM0306 Participates in Host Cell Inflammation Induced by Salmonella enterica Serovar Typhimurium. Ling C, Liang S, Li Y, Cao Q, Ye H, Zhang C, Dong Z, Feng D, Wang W, Zuo J. Int J Mol Sci 24 8170 (2023)
  10. Pathogen genomics and phage-based solutions for accurately identifying and controlling Salmonella pathogens. Lopez-Garcia AV, AbuOun M, Nunez-Garcia J, Nale JY, Gaylov EE, Phothaworn P, Sukjoi C, Thiennimitr P, Malik DJ, Korbsrisate S, Clokie MRJ, Anjum MF. Front Microbiol 14 1166615 (2023)
  11. SPI-1 virulence gene expression modulates motility of Salmonella Typhimurium in a proton motive force- and adhesins-dependent manner. Saleh DO, Horstmann JA, Giralt-Zúñiga M, Weber W, Kaganovitch E, Durairaj AC, Klotzsch E, Strowig T, Erhardt M. PLoS Pathog 19 e1011451 (2023)


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