› 5yat

X-ray diffraction
1.74Å resolution

Crystal structure of mitochondrial alcohol dehydrogenase isozyme III from Komagataella phaffii GS115

Released:
DOI: 10.2210/pdb5yat/pdb
PDB entry 5yat coloured by chain, front view.
PDB entry 5yat coloured by chain, side view.
PDB entry 5yat coloured by chain, top view.
Source organism: Komagataella phaffii GS115
Primary publication:
Investigation of structure and function of mitochondrial alcohol dehydrogenase isozyme III from Komagataella phaffii GS115.
Zhang H, Li Q, Wang L, Chen Y
Biochim Biophys Acta Gen Subj 1862 1199-1208 (2018)
PMID: 29474823

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-504134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alcohol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 37.04 KDa
Source organism: Komagataella phaffii GS115
Expression system: Pichia
UniProt:
  • Canonical: C4R0S8 (Residues: 1-350; Coverage: 100%)
Gene name: PAS_chr2-1_0472
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 4 x GOL
Ligand IPA 2 x IPA
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P64
Unit cell:
a: 111.856Å b: 111.856Å c: 105.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.212
Expression system: Pichia

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