› 5ybh

X-ray diffraction
2.5Å resolution

Structural of the highly conserved ATPase from type III secretion system of bacterial pathogens

Released:
DOI: 10.2210/pdb5ybh/pdb
PDB entry 5ybh coloured by chain, front view.
PDB entry 5ybh coloured by chain, side view.
PDB entry 5ybh coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Structural Insight Into Conformational Changes Induced by ATP Binding in a Type III Secretion-Associated ATPase From Shigella flexneri.
Gao X, Mu Z, Yu X, Qin B, Wojdyla J, Wang M, Cui S
OpenAccess logo Front Microbiol 9 1468 (2018)
PMID: 30013545

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 40.85 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A1C1 (Residues: 84-430; Coverage: 81%)
Gene names: CP0149, mxiB, sctN, spa47, spaL
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand MG 20 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P3221
Unit cell:
a: 105.385Å b: 105.385Å c: 146.386Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.248
Expression system: Escherichia coli

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Citations

3 review citations

The T3SS of Shigella: Expression, Structure, Function, and Role in Vacuole Escape.
Bajunaid et al. (2020)
2 more

2 mentions without citation

Structural Insight Into Conformational Changes Induced by ATP Binding in a Type III Secretion-Associated ATPase From Shigella flexneri.
Gao et al. (2018)
1 more