› 5yd7

X-ray diffraction
1.7Å resolution

Crystal Structure OF VIM-2 Metallo-beta-lactamase

Released:
DOI: 10.2210/pdb5yd7/pdb
PDB entry 5yd7 coloured by chain, front view.
PDB entry 5yd7 coloured by chain, side view.
PDB entry 5yd7 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry author: Wachino J

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 26.14 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K2N0 (Residues: 21-266; Coverage: 100%)
Gene names: PAERUG_P19_London_7_VIM_2_05_10_01672, bla vim-2, blaVIM-2, blm
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand FMT 5 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: C2
Unit cell:
a: 100.8Å b: 79.27Å c: 67.39Å
α: 90° β: 130.15° γ: 90°
R-values:
R R work R free
0.165 0.163 0.213
Expression system: Escherichia coli

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