› 5ye3

X-ray diffraction
1.7Å resolution

Crystal structure of the complex of di-acetylated histone H4 and 2A7D9 Fab fragment

Released:
DOI: 10.2210/pdb5ye3/pdb
Model geometry
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PDB entry 5ye3 coloured by chain, front view.
PDB entry 5ye3 coloured by chain, side view.
PDB entry 5ye3 coloured by chain, top view.
Source organisms:
Primary publication:
JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states.
Handoko L, Kaczkowski B, Hon CC, Lizio M, Wakamori M, Matsuda T, Ito T, Jeyamohan P, Sato Y, Sakamoto K, Yokoyama S, Kimura H, Minoda A, Umehara T
OpenAccess logo Epigenetics 13 410-431 (2018)
PMID: 30080437

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211483 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
2A7D9 VH CH1 chain Chain: B
Molecule details ›
Chain: B
Length: 211 amino acids
Theoretical weight: 22.56 KDa
Source organism: Mus musculus
Expression system: Mus musculus
2A7D9 L chain Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Histone H4 Chain: C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 39.951Å b: 85.978Å c: 122.849Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.195
Expression systems:
  • Mus musculus
  • Not provided

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Citations

3 review citations

Targeting epigenetic regulators for cancer therapy: mechanisms and advances in clinical trials.
Cheng et al. (2019)
2 more

1 mention without citation

JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states.
Handoko et al. (2018)

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