› 5yg1

X-ray diffraction
2.45Å resolution

Plasmodium vivax SHMT bound with PLP-glycine and GS704

Released:
DOI: 10.2210/pdb5yg1/pdb
PDB entry 5yg1 coloured by chain, front view.
PDB entry 5yg1 coloured by chain, side view.
PDB entry 5yg1 coloured by chain, top view.
Source organism: Plasmodium vivax
Entry authors: Chitnumsub P, Jaruwat A, Leartsakulpanich U, Schwertz G, Diederich F

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glycine hydroxymethyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 442 amino acids
Theoretical weight: 49.25 KDa
Source organism: Plasmodium vivax
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5K8L9 (Residues: 1-442; Coverage: 100%)
Gene name: PVX_100730
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments

3 bound ligands:
Ligand PLG 3 x PLG
Ligand 8UU 3 x 8UU
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 101.471Å b: 58.632Å c: 234.636Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.225 0.294
Expression system: Escherichia coli BL21(DE3)

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