› 5yho

X-ray diffraction
2.4Å resolution

Crystal structure of acetolactate decarboxylase from Enterobacter cloacae

Released:
DOI: 10.2210/pdb5yho/pdb
PDB entry 5yho coloured by chain, front view.
PDB entry 5yho coloured by chain, side view.
PDB entry 5yho coloured by chain, top view.
Source organism: Klebsiella aerogenes
Entry authors: Ji FL, Li MY, Feng YB

Function and Biology Details

Reaction catalysed: 
(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-acetolactate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 26.71 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P05361 (Residues: 23-260; Coverage: 92%)
Gene names: aldC, budA
Sequence domains: Alpha-acetolactate decarboxylase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P21
Unit cell:
a: 45.832Å b: 59.981Å c: 80.818Å
α: 90° β: 108.17° γ: 90°
R-values:
R R work R free
0.213 0.21 0.272
Expression system: Escherichia coli

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