› 5yse

X-ray diffraction
1.6Å resolution

Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotetraose

Released:
DOI: 10.2210/pdb5yse/pdb
Model geometry
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PDB entry 5yse coloured by chain, front view.
PDB entry 5yse coloured by chain, side view.
PDB entry 5yse coloured by chain, top view.
Source organism: Listeria innocua Clip11262
Primary publication:
Structural and thermodynamic insights into β-1,2-glucooligosaccharide capture by a solute-binding protein in Listeria innocua.
Abe K, Sunagawa N, Terada T, Takahashi Y, Arakawa T, Igarashi K, Samejima M, Nakai H, Taguchi H, Nakajima M, Fushinobu S
J Biol Chem 293 8812-8828 (2018)
PMID: 29678880

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lin1841 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 44.58 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92AS8 (Residues: 27-414; Coverage: 99%)
Gene name: lin1841
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
2 bound ligands:
Ligand MPD 3 x MPD
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 36.014Å b: 63.28Å c: 87.451Å
α: 89.99° β: 82.24° γ: 86.08°
R-values:
R R work R free
0.159 0.158 0.188
Expression system: Escherichia coli BL21(DE3)

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Citations

6 citation in other articles

Substrate preference of an ABC importer corresponds to selective growth on β-(1,6)-galactosides in Bifidobacterium animalis subsp. lactis.
Theilmann et al. (2019)
5 more

3 mentions without citation

Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis.
Sakanaka et al. (2019)
2 more

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