› 5ysi

X-ray diffraction
1.55Å resolution

Function and Biology Details

Reaction catalysed: 
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitinating/deubiquitinating enzyme SdeA Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 16.92 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZTK4 (Residues: 756-905; Coverage: 10%)
Gene names: lpg2157, sdeA
Sequence domains: SidE mono-ADP-ribosyltransferase domain

Ligands and Environments

1 bound ligand:
Ligand NCA 1 x NCA
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2221
Unit cell:
a: 37.867Å b: 75.698Å c: 84.682Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Non-lysine ubiquitylation: Doing things differently.
Kelsall IR. (2022)
6 more

3 mentions without citation

ADP-ribosylation systems in bacteria and viruses.
Mikolčević et al. (2021)
2 more