› 5yvr

X-ray diffraction
1.9Å resolution

Crystal Structure of the H277A mutant of ADH/D1, an archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase

Released:
DOI: 10.2210/pdb5yvr/pdb
PDB entry 5yvr coloured by chain, front view.
PDB entry 5yvr coloured by chain, side view.
PDB entry 5yvr coloured by chain, top view.
Source organism: candidate division MSBL1 archaeon SCGC-AAA259E19
Primary publication:
Identification and Experimental Characterization of an Extremophilic Brine Pool Alcohol Dehydrogenase from Single Amplified Genomes.
Grötzinger SW, Karan R, Strillinger E, Bader S, Frank A, Al Rowaihi IS, Akal A, Wackerow W, Archer JA, Rueping M, Weuster-Botz D, Groll M, Eppinger J, Arold ST
ACS Chem Biol 13 161-170 (2018)
PMID: 29188989

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 44.3 KDa
Source organism: candidate division MSBL1 archaeon SCGC-AAA259E19
Expression system: Haloferax volcanii
UniProt:
  • Canonical: A0A133UP32 (Residues: 1-400; Coverage: 100%)
Gene name: AKJ65_00115
Sequence domains: Iron-containing alcohol dehydrogenase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand NDP 1 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 58.05Å b: 104.8Å c: 129.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.213
Expression system: Haloferax volcanii

Quick links

Citations

4 review citations

Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods.
Sysoev et al. (2021)
3 more

1 mention without citation

Conservation of binding properties in protein models.
Egbert et al. (2021)